报告题目：Droplet Microfluidics for High Throughput Single-Cell Analysis with Single-Molecule Sensitivity
报 告 人：杨朝勇 教授（厦门大学）
报告时间：2015年5月9日下午2：30
报告地点：化学楼二楼一号会议室
报告人简介：
杨朝勇，男，厦门大学特聘教授、博士生导师、固体表面物理化学国家重点实验室固定成员、厦门大学化学化工学院化学生物学系副系主任。相继于1998和2001年在厦门大学化学系获得学士与硕士学位，2006年获得美国佛罗里达大学博士学位，2006年9月至2007年12月在美国加州大学伯克利分校从事博士后研究。主要研究领域为生物分析化学，在分子探针、微流控芯片、信号放大、单细胞分析、食品安全等方向取得了创新性的成果。 在PNAS、JACS、Angew Chem Int Ed、Anal Chem等学术刊物上发表论文100多篇，H index 31。研究工作被美国Washington Times、The Scientist、英国RSC Chemistry World、欧洲Wiley Chemistry Views、JACS Spotlights、光明日报等媒体与刊物做了专题报道。先后获得了美国化学会分析化学研究生奖、中国政府国家优秀自费留学生奖、中美化学与化学生物学教授协会杰出教授奖、中国青年分析化学家奖、福建省五四青年奖章、福建省运盛青年科技奖、福建省高校领军人才等奖项。2010年获福建省杰出青年科学基金, 2013年获国家基金委杰出青年基金。承担973计划课题1项、国家自然科学基金5项。现任《分析化学》、Analyst、Biomicrofluidics等期刊编、BMC Biochemistry 副主编。
报告简介：
It is well established that individual cells, even from the same origin, differ from each other in many aspects due to stochastic biological processes and differences in environmental perturbations. Cell heterogeneity has been found to play an important role in many biological processes, including cellular differentiation and immune response, as well as disease development. The heterogeneity of cells in culture and in organisms poses a challenge for many experimental measurements. Traditional ensemble analysis based on averaging a large population of cells, as a result, masks the behavior of minority subpopulations and effectively blinding researchers to possibly interesting differences between cells. Single-cell analysis is an important and emerging field that gives insights into heterogeneity between cells and advanced cellular processes at high resolution, which is important for cancer research, regenerative medicine, immune system research and diagnostics, as well as for the production of therapeutics. Microfluidics has proven to be a leading tool for single cell analysis since device dimensions are on the same scale as those of cells, allowing for precise fluid and cell manipulation at high throughput. In this talk, I will present our recent efforts on developing droplet microfluidic technology for high throughput single cell isolation, manipulation, and analysis at the DNA, RNA and protein level with single-molecule sensitivity.
